What It Means To Be Canadian

What It Means To Be Canadian To no ones surprise, being a Canadian means different things to different people and it is quite commonplace for many Canadians to have multiple identities and even multiple allegiances. Predictably, it is not always clear how these multiple identities can fit into Canadian society and fault lines inevitably arise between those with different identities. The next several pages will look at the oldest fault-line of them all at least among Canadians of European extraction which is the fault line between English-speaking Canadians and French-speaking Canadians. It resonates with this writer because, frankly, so much of our constitutional and political history has been wrapped up with trying to resolve the grievances and insecurities of French Canadians. For those within and without this fault line, Canadian identity is complicated because those who fall outside it people who have arrived from Asia or the Caribbean or from various other parts of the world are subtly reminded, through official bilingualism and through the constant constitutional wrangling over whether or not Quebec is a distinct society, that perhaps they are not true or authentic Canadians in the way some other groups are. Further, for French Canadians, the battle has always been between identifying themselves as Canadians or identifying themselves as French-Canadians who deserve to stand apart from other Canadians. This paper will look at the French-English divide in Canada by providing a brief historical overview of the tensions that have long existed between the two sides; as should be plain, the divide has been with us since before Confederation and will surely be with us for some time still to come. The paper will then turn to look at the introduction of Bill 101 in 1977 and how that ushered in a new era of strained English-French relations. With that out of the way, the paper will subsequently observe how the fault line in general has complicated how people who associate with this group identity interact within Canadian society? In short, how have French Canadians (the minority group and the group most likely to be inflamed by linguistic considerations) interacted within Canada in light of the powerful divide that separates them and that exacerbates their hostilities towards one another? With special reference to French Canadians, what does it mean to them (or what has it meant to them rec ently) to be Canadian within the context of Canada? Last of all, the essay will explore what the future of the Canadian national identity might well be should tensions in this fault line increase or tensions in other fault lines increase. We can all imagine that simmering tensions will weaken the connective tissue that binds Canadians together and will create the prospect for the fragmentation of Canadian society unless common ground is found. The only saving grace for Canada with regards to this particular English-French divide is that demographic factors may end up resolving it by changing the composition of Quebec and of Canada so dramatically that the country no longer much cares about English-French hostilities. Historical context of the English-French divide The simple reality is that tensions between English and French have always been a part of the Canadian landscape. In the eighteenth century, the British and French bitterly wrestled for control of North America and, at the end of that century and in the early decades of the next one, there was a significant divide between the French Canadians of Lower Canada and the English elites of that province who deigned to pass measures from on high. Suffice it to say, the educated professional elite that dominated the legislative assembly of Lower Canada from the turn of the nineteenth century onwards reacted most negatively to the disproportionate power held by (and general unresponsiveness exhibited by) the English-dominated colonial executive (executive council) and by the British-appointed governor (Greer, 1993). The end result was the ill-fated and violent 1837 Rebellion in Lower Canada when French-Canadian nationalists finally exploded in armed outrage at the refusal of the British gover nment to seriously contemplate the democratization of the Legislative Council (Breakenridge Read, 2008). As most students of Canadian history are aware, the aforementioned rebellion led to the Durham Report of 1839 wherein John Lambton, the Earl of Durham, advocated the cultural assimilation of French Canadian Lower Canada into a larger union with Lower Canada that would be dominated by the English. In effect, the best way to resolve the sense of grievance percolating in the hearts of French Canadians was to simply assimilate them (Van Male, 1997). For Lord Durham, what was tearing at the entrails of Lower Canada was a profound ethnic and linguistic conflict that fundamentally involved two nations warring in the bosom of a single state (quoted in Greer, 1993, p.153). Ultimately, though tensions did lessen somewhat from their high water mark in the late 1830s, the old animosity never completely went away: at least one observer has written about this tragic element in our historyà ¢Ã¢â€š ¬Ã‚ ¦.this is a country of ingrown prejudicesà ¢Ã¢â€š ¬Ã‚ ¦.unthinking, irrational and mean (Lower Q uoted in Cameron, 1997, p.372). During the subsequent generations, the animus between French Canadians and English Canadians always lurked just beneath the surface and could burst into flame at any moment. In general, many of the most significant moments in Canadian history have either revolved around French-English rapprochement the original constitutional deliberations of the 1860s or have revolved around French-Canadian animosities spilling into the open: the Conscription Crises of Two World Wars; the Richard riots of the 1950s; the Quiet Revolution of the 1960s and the federal governments attempts to head off Quebec nationalism; and the hotly-contested separatist referenda of 1980 and 1995. If one wants to understand the constitutional morass of the 1970s and 1980s (or 1990s) or if one wants to understand the original inspiration for Canadian multiculturalism (for more on how official multiculturalism under Trudeau was chiefly a response to Quebec nationalism, please see Tierney, 2007), then one must understand the fault line between English and French in Canada. Naturally, one of the greatest sources of tension of all was the battle on the part of French Canadians to protect the ir linguistic inheritance from the encroachment of the English majority. Discussion and analysis: how has the divide between English and French, and the formulation of Bill 101, impacted the interactions between the two groups within Canada? The 1977 Quebec language law was probably an inevitable consequence of the Quiet Revolution of the 1960s; protecting French culture from Les Anglais, after all, necessarily meant protecting the language from desecration and from conquest at the hands of English. Specifically, French-Canadian academics at the start of the 1970s wrote that the history of French Canada within the Canadian Confederation was very often a history of fighting to maintain the integrity of the French language. The passage of the Trudeau governments Official Language Law in 1970 saw French recognized as an official language in all federal affairs and constituted a victory of sorts, but the corresponding (and rather surprising) efforts of the Quebec government to pass Bill 63 a bill that would have granted the English language official status in Quebec was perceived as a direct threat to the primacy of the French language and viewed as setting the groundwork for the anglification of the population of Quebec ( Angers, 1970). Obviously, this raised the temperature in the room when it came to the ongoing debate about what measures should be taken to protect the French language in Quebec and expedited the arrival of Bill 101. The fault line between French Canada and English Canada has impacted or complicated how both groups (but particularly French Canadians) interact with Canada and with their Canadian identity in the sense that it has created a hyphenated group of Canadians who can be reliably expected to break down on the issues according to their linguistic background. The great conscription crises and the animus unleashed in the two referendum campaigns nearly a generation apart attest to how people on both sides (but especially French Canadians) have elected to define themselves by the language they speak than by the country of which they are a part. Bill 101, maybe more than any other single piece of legislation, reminded all Canadians of how the fault line between English and French was predicated upon concerns over language and, specifically, whose language would survive over time. Examining the act itself, Bill 101 was an act that mandated a number of things that could only have heightened the mistrust and paranoia of the English-speaking minority in Quebec at the same time as it surely disenchanted new arrivals from elsewhere in the world. Notably, Bill 101 decreed that French-only public signs were to be a feature of the province; French became the language of work in public institutions; and the autonomy of English schools in Quebec was sharply reduced (Levine, 1990). And, as most students are aware, and as our course notes remind us, Bill 101 also mandated that all students receive their schooling in French. The bill was a shot across the bow of English Quebec and divided Canadians dramatically along ethic and linguistic affiliation. To get to the heart of the matter, for French Canadians, Bill 101 was simply a re-conquest that merely asserted that French was the dominate language of la Belle Province; for English-speaking Quebeckers, however, the passage of Bill 101 was a clear repudiation of the English language as it stripped away the Charter status of the English language and also limited the rights and privileges of a linguistic group that, historically, had wielded most of the power in Quebec (Levine, 1990, p.119). Now, and maybe forever after, the centrality of language to ones conception of his or herself and his or her place in Canada could no longer be swept under the rug and the pretence that we were/are all loyal Canadians first was shattered. In general, Bill 101 has allowed the French language to retain somewhat of its lustre amongst visible minorities arriving in Quebec: recent data compiled by the Canadian Human Rights Commission indicates that, by a 2 to 1 margin, French is the first official language of visible minorities in the province (Canadian Human Rights Commission, 2007). Source: Canadian Human Rights Commission. (2007). Strategic Initiatives: section 6 Quebec. Retrieved August 5, 2010 from http://www.chrc-ccdp.ca/proactive_initiatives/bvm_mvb/page6-en.asp Information such as that above indicates that any hopes of complete English conquest of Quebec will have to wait for a little while longer. In fact, a closer look at the data reveals that the number of Quebeckers who identify English as their Mother Tongue appears to be declining and has been for several years (Office of the Commissioner of Official Languages, 2007). Source: Office of the Commissioner of Official Languages. (2007). Ethnolinguistic composition of the population. OCOL. Retrieved August 5, 2010 from http://www.ocol-clo.gc.ca/html/ar_ra_04_05_v1_14_e.php We can safely conclude that language laws have contributed, even if indirectly, to the exodus of English speakers out of Quebec and to the polarization of sentiments between English and French within Quebec. However, such language laws do not guarantee the future of the French language in the province given the mass influx of new Canadians who speak neither English nor French or who are disinterested in learning French. For many French Canadians, being Canadian may still mean being French Canadian first above all else; however, as the demographic shape of Canada changes due to high immigration, they may find themselves even more isolated than ever before but this time isolated within a huge polyglot nation where the competing languages are not just English but dozens, or even hundreds, of others. At the end of it all, any increased tensions between French and English in Canada will tear at the Canadian national identity in the sense that it undermines the legitimacy of the confederation to have the two founding languages fighting with one another. On the other hand, even if simmering tensions will only intensify the self-identification of French Canadians with their French heritage, the reality is that all the chauvinism in the world may not matter chiefly because French Canada and English Canada are becoming relatively smaller pieces of the Canadian mosaic as the nation welcomes in people from Asia, from Africa, from South America and from Eastern Europe who do not have either language as their first language. Ultimately, if other divides in Canada fall the French-English model and grow more acrimonious, then the countrys future could be at risk; however, the French-English divide will probably become less important over time. Conclusion The past several pages have looked at the English-French divide in Canada, the ancient fault-line, and have argued that language laws instituted in Quebec surely did not help in bringing the two sides together; if anything, ancient animosities were revived. However, Canada is a changing nation and that means that no one can safely assume that Canada will tear apart if the gulf between English and French widens. The future is uncertain, but it is unlikely that the French and English divide will remain the dominant one in Canadian life simply because Canada is a country that is moving beyond its French/English past.

Biology Lab

Lab   #1   ? Introduction   to   the   Microscopy   & Observation   of   Prokaryotic   and   Eukaryotic Cells Introduction Many   of   the   cells   and   organisms   that   you   will   be   studying   are   at   the   lower   limits   of   visibility   of   light   microscopes;   therefore,   it   is   extremely   important   that   you   attain   critical   lighting   and   focussing. It   is   also   important   to   handle   the   microscope   competently   to   avoid   damaging   either   the   microscope   or   the   preparation   you   are   studying. Even   students   who   have   previously   used   microscopes  should  read  the  instructions  carefully. Guide  Biolabo Using a web rowser, go to   the   following   web   site: http://salinella. bio. uottawa. ca/biolabo/   (you   can   try   it   from   home). Under   Microscopy   you   will   find   links   to   pages   that   describe   both   type   of   microscopes   you   will   use   this   semester,   as   well   as   how   to   set   up   and   use   them. It   is   strongly   recommended   that   you   visit   these   pages   prior   to   attending  your  first  lab. Image  J  /  Qcapture Although   you   can   make   all   your   observations   by   watching   directly   through   the   oculars,   it   also   can   be   done   on   the   computer   screen   using   the   digital   camera   attached   to   each   microscope.For   that,   you   will   use   the   Image   J   program   together   with   a   capture   plugin called   Qcapture. Visit   the   lab   website   to   learn   how   to   use   Image   J   (link   on   the   homepage). All   observations   can   be   made   on   your  computer   screen   or   in   the   oculars. Each   method   has   its   advantages   and   drawbacks;   you   will   have   to   choose   which   one  it  more  appropriate  (or  the  one  you  prefer): Oculars Screen ? Greater  resolution ? Wider  field  of  view ? Can  share  observation  with  others ? More  comfortable  for  users ? Take  pictures  while  observing Lab1  ? Microscopy The  Compound  Microscope On   the   Guide   Biolabo   page   click   on   the   CX41   Compound   Microscope   link   then   on   Parts   and   Function. This   will   bring   up   a   labelled   line   diagram   of   your   microscope. Familiarize   yourself   with   the   various   components   shown   in   this   figure. Then,   click   on   Setup   and   Bright   field   alignment   in   order   to   know  how  to  use  and  handle  the  microscope. Now,   locate   your   compound   microscope   in   the   cupboard   below   the   sink   of   your   workstation. Place   it   on   the   counter   between   the omputer   and   the   end  of  the  counter. Be  sure  that  whenever  you  transport  the  microscope,  it   is   always   kept   upright;   the   ocular   lens   will   fall   out   if   the   scope   is   tilted   or   swung. Even   though   you   don’t   need   the   dissecting   microscope   right   now,   take   it   out  of  the  cupboard  and  install  it  beside  the  compound  microscope. Connect   one   firewire   cable   to   each   of   the   cameras   installed   on   top   of   the   microscopes. This  way,  everything  is  setup  for  further  observations  both  on   your  computer  screen  and  through  the  oculars. Parts  of  the  compound  microscopeThe   microscope   consists   of   a   system   of   lenses,   a   light   source,   and   a   geared   mechanism   for   adjusting   the   distance   between   the   lens   system   and   object   being   observed. There   are   a   number   of   important   components   and   it   is   essential   that   you   be   able   to   identify   them   and   understand   their   function   before  you  can  proceed. By  going  through  the  different  modules  in  Biolabo   and   using   the   microscope   you   will   develop   a   competency   for   bright   field   microscopy. Identify   the   following   components   using   Biolabo   (Parts   andà ‚   functions   figure)  and  your  microscope:REVOLVING  NOSEPIECE:  Supports  the  various  objectives  ? You  will  only  use   the  4x,  10x  and  40x  objectives  in  the  BIO1140  labs  (not  the  100x). STAGE:   Supports   the   specimen   being   observed. A   system   of   knobs   on   the   side   of   the   stage   allows   you   to   move   the   specimen   under   the   objective   on   the  X  and  Y  axes. Try  and  move  the  stage. COARSE   FOCUS   KNOB:   Permits   rapid   change   in   distance   between   the   specimen   and   the   objective   thereby   allowing   for   rough   focussing   –   Do   not   use  when  focusing  with  the  40x  objectiveFINE   FOCUS   KNOB:   Permits   small   changes   in   distance   between   the   specimen   and   the   obje ctive   and   thereby   allows   for   final   focussing   of   the   image. 10 Lab1  ? Microscopy OCULAR   OR   EYEPIECE:   A   magnifying   element   in   the   microscope,   usually   10X. It   is   through   the   ocular,   or   eyepiece   that   one   looks   at   the   specimen. All   our   microscopes   are   parfocal,   so   that   when   an   object   is   in   focus   with   one   objective,   the   focus   will   not   be   completely   lost   when   changing   to   the   next  objective. OBJECTIVES:  The  magnifying  element  which  is  closest  to  the  specimen.See   figure  1  to  find  out  about  the  engravings  on  the  side  of  each  objective. CONDENSER:   System   of   lenses   that   concentrates   the   light   furnished   by   the   illuminator. It  does  not  magnif y  the  object. CONDENSER   HEIGHT   ADJUSTMENT   KNOB:   Allows   one   to   focus   the   concentrated  light  onto  the  specimen. APERTURE  IRIS  DIAPHRAGM:  Used  to  reduce  glare  from  unwanted  light  by   adjusting  the  angle  of  the  cone  of  light  that  comes  from  the  condenser; Production  of  Image  by  a  Compound  Microscope The   most   important   part   of   a   microscope   is   the   objective.All   the   other   parts  of  the  instrument  are  designed  to  help  the  objective  produce  the  best   possible  image. The  best  image  is  not  the  largest;  it  is  the  clearest. There  is   no   value   to   a   high   magnification. If   the   resolution   is   poor   you   will   have   no   better  understanding  of  the  specimen. light  beam ocularà ‚  lens Magnification Numerical  aperture  (NA) Determines   the   resolving power  of  the  objective* Optical   tube   length   /   max. coverslip  thickness  in  mm prism objective  lens specimen condenser  lens Figure  1:  Objectives  engravings light  sourceFigure  2:  Image  production  in  a  compound  microscope. 11 Lab1  ? Microscopy *Resolving  power  is  the  ability  to  see  two  objects  that  are  very  close  as  two   separate  objects. The  human  eye  will  resolving  power  is  about  100 µm. Using  the  compound  microscope Always   handle   the   microscope   GENTLY! It   is   an   expensive,   delicate   and   heavy   instrument. Carry   it   with   two   hands,   one   hand   on   the   arm,   and   the   other   hand   under   the   base. If   the   ocular   or   objective   is   dir ty,   wipe   it   clean   using   ONLY   Kimwipes   or   special   lens   tissue   and   cleaning   fluid   supplied.If   you   use   anything   else   you   may   scratch   the   lens. Wipe   up   any   cleaning   fluid   immediately;   otherwise   it   will   dissolve   the   glue   which   holds   the   lens   in   place. REMEMBER,  your  demonstrator  is  here  to  help,  so†¦ ASK! 1. Make   sure   that   the   power   cord   is   plugged   into   the   back   of   your   microscope  and  into  a  power  outlet. 2. Using  the  letter  Ã¢â‚¬Å"e†Ã‚  microscope  slide  provided,  follow  steps  2  through  13   in   the   Setup   and   Bright   field   alignment   procedure   of   Biolabo. Remember,   observation  can  be  done  on  screen  or  through  the  oculars. Orientation  an d  working  distance . Starting   your   examination   with   the   4X   objective,   position   the   letter   â€Å"e†Ã‚   slide  on  the  stage. 2. Draw  what  you  see  in  the  microscope:_________________ 3. What  would  a  slide  with  the  letter  Ã¢â‚¬Å"t†Ã‚  look  like  under  the  microscope? _________________ 4. Using  the  knobs  located  on  the  side  of  the  stage  and  looking  through  the   microscope,   move   the   slide   slowly   to   the   right,   then   to   the   left. Record   your  observations. ___________________________________   5. Now,   move   the   slide   slowly   away   from   you,   then   towards   you   while   observing  through  the  microscope.Record  your  observations   ____________________________________ 6. Focus  on  the  slide  at  10X. Check  the  dist ance  between  the  objective  lens   and  your  slide  (=  the  working  distance,  see  also  the  reference  at  the  end  of   this   chapter). Now   switch   to   the   40X   objective   and   check   the   working   distance. What   happens   to   the   working   distance   as   your   magnification   increases? 12 Lab1  ? Microscopy Depth  of  field  (depth  of  focus) Lenses   have   a   depth   of   focus. It   is   the   number   of   planes   in   which   an   object   appears   to   be   in   focus.Extend   your   fist   at   arm’s   length   in   front   of   you   and   hold   your   thumb   up. Concentrate   on   your   thumb   and   notice   that   the   objects  past  your  thumb  on  the  other  side  of  the  room  are  not  clearly  seen. Similarly   withà ‚   a   microscope,   when   it   is   focussed   on   one   surface,   the   surfaces  lower  or  higher  will  be  out  of  focus. 1. Position   a   prepared   slide   with   coloured   threads   upon   the   stage. At   low   power,  4X,  focus  on  the  area  where  the  threads  cross. 2. Using  the  fine  focus  adjustment,  focus  up  and  down  slowly. 3. Repeat   using   different   objectives.What   can   you   say   about   the   depth   of   field   at   different   magnifications? Has   it   increased   or   decreased? (i. e. ,   can   you  see  more  threads  in  one  focal  plane  at  4X  or  40X? ) ____________________________________________________________ Magnification The  magnification  given  by  objectives  and  oculars  is  engraved  on  them. The   total   magnification   for   any   combination   of   objective   and   ocular   is   the   product  of  the  magnification  of  each  lens. Objective  magnification Ocular  magnification Total  Magnification Light  intensity Working  distance 4x 10x 40x High 22mm 10x 10x 100xMedium 10. 5mm 40x 10x 400x Low 0. 56mm Table1   . Comparison   magnification,   working   distance   and   brightness   at   three   different   objective  magnifications. You  also  can  calculate  the  magnification  of  your  picture  using  the  following   formula: Magnification  factor=  measured  size  of  object  =  (  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  X) Actual  size  of  object 13 Lab1  ? Microscopy Specimen   size   and   Magnification   of the  picture Before   you   start   this   exercise,   make   sure   you   have   carefully   read   the   website   section   rele vant   to   the   software   you   will   use   to   take   digital   pictures  (ImageJ/Qcapture).The   goal   of   this   section   is   to   teach   you   different   techniques   that   will   allow   you   to   determine   the   size   of   objects   you’re   observing   under   the   microscope. The   general   principle   is   fairly   simple:   2   objects   have   the   same   relative   size   (expressed   as   a   ratio)   in   the   real   world   and   under   the   microscope. actual  size  of  object  A  Ã‚  Ã‚  =  on? screen  size  of  object  A  Ã‚  ? A1  =  A2     actual  size  of  object  B  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  on? screen  size  of  object  B  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  B1  Ã‚  Ã‚  Ã‚  B2 The  following  exercises  are  applications   of  this  formula. Place  a  sli de  under   the   microscope.Choose   the   right   objective   and   adjust   the   focus   and   light   level. Then,  choose  a  structure  you  want  to  measure  and  take  a  picture. A? First   method:   Measuring   an   object   using   the   field   of   view  (FOV): The   simplest   way   to   determine   the   size   of   an   object   is   to   use   the   known   size  of  the  whole  field  of  view  (FOV,  the  whole  picture  from  left  to  right). 1? On   the   computer   screen   (using   a   ruler   and   without   writing   anything   of   the  screen),  measure  the  object  of  which  you  want  to  determine  the  size  (=   A2) 2? Then,  measure  the  width  of  the  whole  picture  on  the  screen  (=B2). ? Refer   to   table   2   on   page   20   to   know   the   actual   size   of   the   field   of   view   for  the  objective  you’re  using  (=B1) 4? Use  the  following  formula: Actual  size  of  the  object  (A1)  =  Actual  size  of  the  FOV  (B1)  Ã‚  Ã‚  Ã‚  Ã‚  x  Ã‚  Ã‚  on? screen  size  of  the  object  (A2) on? screen  size  of  the  FOV  (B2) Example:   On   a   snapshot   using   the   4x   objective,   an   insect   has   an   on? screen   length  of  10cm. The  whole  picture  is  20cm  wide. What  is  the  actual  size  of  the  insect? ______________________________ 14 Lab1  ? Microscopy B? Second   method:   Measuring   an   object   using   a   scale   bar  file:From   Image   J   (using   the   file   /   open   command),   open   the   file   that   contains   the   relevant   scale   bar   in   the    (T:/BIO/BIO1140):   new10X. jpg   for   the   10x   objective,  and  new40X. jpg  (for  the  4x  and  40x  objectives). Then,   using   a   ruler   measure   the   following   distances   directly   on   the   computer  screen: 1? The   on? screen   length   (or   width)   of   the   object   whose   size   you   wish   to   determine  (=A2) 2? The  width  of  the  scale  bar  on  the  screen  (=B2)   You  now  can  calculate  the  actual  size  of  the  object  using  the  formula: actual  size  of  object  =  on? creen  length  of  object  Ã‚  x  Ã‚  actual  size  of  scale  bar*        Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  on? screen  length  of  scale  bar ?  Ã‚  Ã‚  Ã‚  A1  =  A2  x  B1 B2 *The   actual   size   of   the   scale   bar   is   indicated   on   the   scale   bar   file   (ex:   on   the   new10x. jpg   file,  the  bar  represents  0. 2mm  at  10x  or  0. 02mm  at  100x)  =  B1 Example:  I  took  a  picture  of  a  small  insect  larva,  using  the  4x  objective. The   larva   length   is   60mm   on   the   screen. The   scale   bar   on   the   new40x. jpg   is   30mm  and  represents  0. 2mm. What  is  the  actual  size  of  the  larva? _________________________Do  not  put  the  compound  microscope  back  in  the  cupboard  you  will  need  it   later  this  afternoon. Points  to  remember  concerning  microscopes 1. Always   work   with   a   clean   microscope. Use   only   the   lens   paper   provided. Don't  forget  to  clean  the  slide  too! 2. Always   locate   the   specimen   under   low   power   and   work   your   way   up   to   the  high  power  objective. 3. Never   use   the   coarse   focusing   knob   when   the   high   power   lens   is   in   position. Use  only  the  fine  focus  knob. 4. Never  use  the  100x  in  1st  year  labs  (we  didn’t  teach  you  how)   5.Always   readjust   illumination   whenever   you   change   the   objective. Too   much  light  will  give  you  a  blurry  image  that  you  cannot  focus  on. 15 Lab1  ? Microscopy The  stereoscopic  microscope (dissecting  microscope) The   stereoscopic   microscope,   also   called   stereoscope   or   dissecting   microscope,   is   used   to   view   objects   that   are   too   large   or   too   thick   to   observe  under  the  comp ound  microscope. Stereo   microscopes   are   always   equipped   with   two   oculars   producing   a   stereoscopic   or   three? dimensional   image. Unlike   the   compound   microscope,  the  image  is  not  inverted.Our   stereo   microscopes   provide   magnification   in   the   range   of   6. 7X   ? 45X   using   a   zoom? type   lens   system. By   rotating   a   dial   located   on   the   right   side   of   the   stereo   microscope   head,   the   viewer   obtains   a   continuous   change   of   magnification. Our   stereo   microscopes   can   be   used   with   reflected   or   transmitted   light. Reflected   light   is   directed   unto   opaque   specimens   from   above   and   is   reflected   to   the   viewer. Transmitted   light   is   used   with   translucent   specimens   and   passes   through   the   specimen   from   beneath   the   stage   and   into  the  viewer's  eyes.Use  of  the  stereoscopic  microscope 1. On   the   Biolabo   home   page   left   click   on   Stereoscope   (Dissecting   microscope)  and  then  on  Stereoscope  setup. 2. Click  on  Step  1  and  read  it  carefully. Obtain  a  stereo  microscope  from  the   same  cupboard  as  your  compound  microscope  if  you  haven’t  yet. 3. Click  on  and  read  steps  2  through  7. 4. Place  a  coin  on  the  stage. 5. Using   the   focussing   knob   on   either   side   of   the   arm,   lower   or   raise   the   objective   until   the   coin   is   in   focus. Examine   it   in   both   reflected   and   transmitted  light.Which   is   best   for   an   opaque   specimen? Try   t he   various   magnifications   by   turning   the   zoom   knob. The   reflected   light source   is   similar   to   a   spotlight   and  its  orientation  can  be  adjusted  manually. Try  rotating  the  light  upwards   and  downwards. 6. Examine  other  materials  such  as  brine  shrimp  larvae  (Artemia)  in  a  watch   glass   using   both   reflected   and   transmitted   light. Add   1? 2   drops   of   â€Å"proto? slow†Ã‚   solution   to   slow   down   the   larvae. Estimate   the   actual   size   of   one   larva:  __________ 16 Lab1  ? Microscopy Prokaryotic  and  Eukaryotic  cellsIt   has   long   been   recognized   that   living   organisms   are   composed   of   basic   structural   and   functional   units   called   cells. Cells   can   be   divided   into   two   general   type s:   prokaryotic   and   eukaryotic,   based   on   the   presence   of   a   nucleus  and  other  membrane  bound  organelles  in  the  latter. Prokaryotic   cells   belong   to   2   big   groups:   archaea   and   eubacteria. They   are   usually   smaller   than   eukaryotic   cells   (typically   1? 5 µm). These   unicellular   organisms  may  be  small,  but  they  are  the  most  abundant  organisms  on  the   planet,   representing   about   half   the   biomass   (Biology,   Brooker   et   al. 010,   McGraw? Hill&Ryerson). They   are   devoid   of   membrane   bound   organelle   such   as   the   nucleus,   mitochondria   or   chloroplasts. Their   genetic   material   is   usually   composed   of   one   circular   chromosome   plus   other   extra   chromosomal  elements  called  plasmids. Euka ryotic   cells   are   usually   much   larger. They   possess   a   membrane   bound   nucleus,   their   organelles   are   more   complex   and   numerous,   and   their   genome   is   larger   than   prokaryotes. Eukaryotic   organisms   can   be   uni? or   multicellular. You   will   have   a   chance   to   observe   many   eukaryotic   cells   during  this  semester:  Amoeba,  Lilly,  Whitefish†¦.In   today's   exercise   you   will   take   a   first   look   at   the   similarities   and   differences   between   prokaryotic   and   eukaryotic   cells   as   well   as   the   diversity  within  these  groups. You   should   familiarize   yourselves   with   a   whole   array   of   cellular   structures   and   organelles   you   will   probably   encounter   during   the   course   of   this   exercise. Before   your   scheduled   lab   session,   write   down   the   definition   and   function  for  each  of  the  following  terms:  plasma  (cell)  membrane,  cell  wall,   protoplast,  cytoplasm,  vacuoles,  nucleus,  nucleolus  and  chloroplasts.Eukaryotic  Cells:  Elodea  (plant) 1? Get   a   young   green   Elodea   leaf   from   the   jar. Mount   it   in   a   drop   of   water   on   a   clean   microscope   slide   with   the   convex   side   of   the   leaf   uppermost. Cover  the  preparation  with  a  coverslip. 2? Observe   the   preparation   at   4X,   then   at   10X. If   you   see   brownish   oval   structures   on   the   leaf   surface,   ignore   then. These   are   probably   epiphytic   diatoms. Concentrate  your  attention  on  the  cells  near  the  cent ral  rib  at  the   base  of  the  leaf  and  on  the  marginal  cells  at  the  edge  of  the  leaf. Can  you  distinguish  several  layers  making  up  the  leaf? ____ ? What   is   the   average   length   ______   and   width   ______   of   the   cells   in   micrometres? 17 Lab1  ? Microscopy 3? Focussing  at  40X  locate  the  cell  wall,  the  vacuole,  the  cytoplasm  and  the   numerous  green  chloroplasts. ? What   important   biological   process   takes   place   in   the   chloroplasts? _____________________________________ ? What  pigment  is  responsible  for  their  green  colouration? ________________________________________________ ? What  is  the  shape  of  chloroplasts? ____________________________________________ ? Are  the  chloroplasts  moving? What  sort  of  movement? ____________________________ _____________________ ? The  phenomenon  you  are  observing  is  called  cytoplasmic  streaming   or   cyclosis. What   do   you   think   the   function   of   such   a   process   could   be? ___________________________________________________ 4? You   have   probably   realised   that   the   plasma   membrane   cannot   be   seen   in   plant   cells. It   is   too   thin   to   be   resolved   with   the   compound   microscope.In   order   to   see   the   true   limiting   boundary   of   the   cytoplasm   it   is   necessary   to   treat   the   cells   in   such   a   manner   that   the   plasma   membrane   becomes   withdrawn   away   from   the   rigid   cell   wall. This   can   be   done   by   placing   the   cell   in   a   strong   salt   solution. This   will   cause   water   toà ‚   diffuse   out   of   the   cell   by   osmosis,   thereby   decreasing   the   cell   volume. The   unaffected   cell   wall remains  in  its  original  state. What  can  then  be  seen  is  a  space  between  the   cell  wall  and  the  limiting  boundary  of  the  protoplast  (the  cell  minus  the  cell   wall)  which  thereby  becomes  visible. Remove   your   Elodea   slide   from   the   microscope   stage. Delicately   remove   the   coverslip,   add   one   drop   of   5%   NaCl   solution   then   put   back  the  coverslip  on  your  preparation ? Refocus   at   40x   (don't   forget:   you   must   first   focus   at   4X,   then   10X   and  finally  at  40x). ? Are   the   cells   plasmolyzed? (If   not   wait   a   while   longer). How   do   they   look  like  now? __ ____________________ ? Has  the  cell  wall  been  affected? _________________   ? What   becomes   of   the   large   central   vacuole   during   plasmolysis? ______  _______________________________________________ Take  a  picture  of  a  plasmolyzed  Elodea  cell. How  does  it  compare  to   the  previous  picture? 18 Lab1  ? Microscopy Prokaryotic  Cells:  Lyngbya  (eubacteria:  cyanobacteria)   1. Take   a   close   look   at   the   sample   in   the   jar. Which   colour   would   best   describe  its  appearance? ___________________ 2. Prepare  a  wet  mount  of  fresh  Lyngbya  by  the  following  procedure:   ? With   forceps   or   an   eye   dropper,   put   a   very   small   amount   of   green   matter  on  a  clean  slide ? Add  a  drop  of  water  from  the  jar. ? Carefully   place   a   co verslip   over   it. Make   sure   it   lies   flat   on   the   preparation.Don't   worry   if   there   are   just   a   few   air   bubbles. With   practice,   your   skills   will   improve. However,   if   too   many   air   bubbles   are   present,   your   preparation   risks   to   dry   out   very   quickly   during   viewing,  compromising  your  observations. 3. Starting  with  the  4X  objective,  focus  on  your  preparation. ? Can  you  see  numerous  green  filaments? _______ ? Are  the  filaments  moving? __________ 4. Switch  to  the  10X  then  the  40X  objective  and  focus  using  the  fine  focus   knob  only: ? Do  you  see  the  individual  cells  making  up  each  filament? ________ ? Estimate  the  width  of  one  filament  in  micrometres:_______ What’s  the  filamentà ‚  width  in  millimetres  (mm)? ________ ? REMEMBER:   You   are   working   with   living   cells. Work   quickly   and   keep   your   specimen   wet   at   all   times. Dead,   dry   or   damaged   biological  preparations  are  useless. Returning  the  microscopes  after  use After   completing   all   observations,   turn   and   click   the   low   power   objective   (4X)  on  the  compound  microscope  into  position. Remove  the  slide  from  the  stage  and  return  it  to  its  correct  box. Wipe  the  stages  with  a  clean  paper  towel. Carefully  disconnect  the  camera  from  the  firewire  cable.Make   sure   you   turned   off   the   light   on   each   microscope,   then   unplug   the   power  cord  and  make  a  loose  coil  of  it  around  the  eyepieces. Return  the  microscope  in  the  cupboard. 19 Lab1  ? Microscopy TAs   will   check   that   you   properly   returned   the   microscopes   in   the cupboard   with  the  cord  properly  attached  and  no  slide  present  on  the   stage. You   will   lose  marks  for  this  lab  (and  other  labs)  if  you  don’t  do  so. Evaluation A   short   quiz   on   microscope   components,   specimen   observations   and   measurement  of  objects  will  take  place  at  the  beginning  of  Lab2.Be  on  time,  the  quiz  will  start  at  2:30. References: 1? Metric  system  (see  also  appendix  IV  at  the  end  of  lab  manual):   1  centimetre  cm  =  10? 2  metres  (m) 1  millimetre  mm  =  10? 3  metres 1  micrometre  ? m  =  10? 6  metres 1  nanometre  nm  =  10? 9  metres 2? Size  of   camera  field  of  views  (fov): Table  2:  Fields  of  View:  Olympus  CX41  Compound  Microscope   Objective 4X 10X 40X 100X Camera  field  of  view (width  in  mm) 1. 75 0. 70 0. 175 0. 070 Table  3:  Fields  of  View  Ã¢â‚¬â€œÃ‚  Olympus  SZ61TR  Dissecting  Microscope   Zoom  Setting 0. 67X 0. 8X 1X

Napoleon Bonaparte Rise to Power Essay

In 1784 to 1785 Napoleon attended the Ecole Militaire in Paris. That was the place where he received his military training. He studied to be an artillery man and an officer. He finished his training and joined the French army when he was 16 years old! Soon after that his father died and he was left with the responsibility of taking care of the huge Bonaparte family. Napoleon was stationed in Paris in 1792. After the French monarchy was overthrown on August 10, 1792, Napoleon decided to make his move up in the ranks. After this, Napoleon started becoming a recognized officer. In 1792 Napoleon was prompted to the rank of captain. In 1793 he was chosen to direct the artillery against the siege in Toulon. He seized ground where he could get his guns in range of the British ships. Soon after that Toulon fell and Napoleon was promoted to the rank of brigadier general. In 1795 he saved the revolutionary government by dispersing a group of rioting citizens by using his famous â€Å"Whiff of grapeshot† – He loaded a bunch on pellets into a cannon and fired it at the crowd. Napoleon was made commander of the French army in Italy. He defeated four Austrian generals in succession, and each army he fought got bigger and bigger. This forced Austria and its allies to make peace with France. But after this Napoleon was relieved of his command. He was poor, he was suspected of treason, he had no friends. No one would have suspected what Napoleon would do next. In 1795 Napoleon was appointed to put down a revolt in Paris. He calmly took complete control of the situation. He just had his men shoot all the rebels in the streets. The French government was saved, but they decided to form a new government called the Directory. Under the new government Napoleon was made commander of the French army in Italy. During this campaign the French realized how smart Napoleon was. He developed a tactic that worked very efficiently. He would cut the enemy’s army in to two parts, then throw all his force on one side before the other side could rejoin them. This method was extremely effective against the Sardinian troops, because he defeated them five times in 11 days!! This made the King of Sardinia to try to make peace. Napoleon could not be stopped. He was a fast thinker who moved his troops extremely fast. Soon, instead of taking the defensive position Napoleon started taking the offensive position and thus, he started his conquest of Europe. He started his attack on Austria. It was his first big campaign. During one attack he showed his bravery by forcing his way across a burning bridge. After that his troops gave him the name Petit Caporal† or in English â€Å"Little Corporal† the name stuck to him. He then attacked the Austrians in Mantua. Austria sent troops there four times, and every time Napoleon crushed them. In 1797 he came within 80 miles of Vienna when Austria surrendered. Napoleon had won 14 pitched battles and 70 combats. He had made the rich lands he conquered feed and pay the French soldiers. Plus millions of francs were send back to France. This helped France’s poor economy tremendously. Napoleon negotiated a treaty called Campo Formio with Austria. Austria gave up it’s Netherlands and Lombardy to France. Austria also recognized the Rhine as the eastern boundary of France. In return France gave Austria most of the old Venetian Republic. When Napoleon returned to Paris he received a huge welcome. He then began thinking of pursuing political power and military power. He wanted to become the next Alexander the Great, so he asked the Directory if he could take a large army to Egypt. That way he could conquer an empire that included Egypt, India, and other middle and far east places. Napoleon came up with a neat idea to accomplish this. If he conquered Egypt, he could attack the English’s route to India. He won the battle of the Pyramids in July 1798. But his fleet was destroyed at the Battle of the Nile in Aboukir Bay. So Napoleon decided to invade Syria. The English and Turkish troops in Syria had held up against Napoleon. Napoleon then retreated to Egypt. Then later in July 1799, he defeated 10,000 Turks at Aoukir. He returned to France shortly after. Napoleon returned to find the Directory was a mess. He, in his selfish way, saw this as the perfect time for self-advancement. So in November of 1799 he overthrew the Directory. Napoleon set up a government called the Consulate. He was the first of three consuls. About three years later he made himself first consul for life. Everyone in France loved Napoleon at that time. Then he started increasing his power Napoleon’s impact is still around today. Many laws are based on the Napoleonic Code. In Paris there are many monuments saluting him. His remains were brought to Paris in 1840 and placed in the Invalides, where they still lie.

Problems Afflicting the Native Americans - 1991 Words

The American Indians are still fighting for more benefits and rights. To get a realistic impression of the Native Indians, it is absolutely necessary to look at them from all the sides and to realize their problems. Though the reservations in the USA, in fact, are on a different level of development. The problems of the Native Americans are varying and of different graveness. But one problem produces the next, in many cases. So there is no shortage of the worrying aspects among the American Indians definitely in the reservations as well as outside. There are only 52 million acres left today from the original American Indian homeland of about 6.1 billion acres which form North America. This land is mostly of inferior quality. The Bureau of†¦show more content†¦The Social challenges In and outside the reservations the Native Americans have to deal with more worrying social developments. All the ethnic groups in the USA the American Indians have the highest rate of school drop outs (about 54%),the highest rate of suicide, the highest rate of teenage pregnancy, the highest rate of teenage suicide, the highest rate of child mortality and lowest life expectancy. The drug abuse and the alcoholism have become a very common problem among the American Indians. Even in some reservations eight families out of ten have the problems with alcoholism. Most unfortunately it spreads among their children too. For those the problem with the unemployment, the decay of the reservations, environmental destruction and the lack of any positive future prospect are probably very hard to bear. Being promoted by the drug abuse, there are a lot of crimes in the reservations and outside also. The American Indians, especially the children, are becoming victims and offenders. At the same rate the domestic violence, child abuse, rape and child neglect are reported to take place regularly in the reservations. The estimated number of the unknown cases is also very high. In the recent years the gang violence in the reservations has increased a lot. It is fuelled by the weak law enforcement. The youth unemployment and the lack of activities for the young Indians result in vandalism, sexual assaults, theft and streetShow MoreRelatedKnowledge Gained Outside of a Formal Education Essay554 Words   |  3 Pagesand blindness that was afflicting the black race in America.† Being in prison, there was not much to do except read. His period in solitude and indiscriminate literary choices led to a better understanding of the extent of his race’s lack of education. Knowledge gives individuals a chance to become more than what society expects of them. By knowing more, people are set free from superficial assumptions that they have of things. 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Illegal immigration causes many problems in the United States, including economic problems, crime, education disputes, and overcrowding. All of these problems were already damaging our country and illegal immigration has made them much, much worse. Let me begin to explain the problem with a story. A woman named Xiomara T. escaped Nicaragua in 1991 to escape the violence afflicting her country (Jost, Feb. 3, 1995). She slipped across the Mexican-American border easily one night without papersRead More Residential Segregation and Social Justice Essays1910 Words   |  8 Pagesacross the country, America’s neighborhoods remain highly segregated along racial and ethnic lines. Residential segregation, particularly between African-Americans and whites, persists in metropolitan areas where minorities make up a large share of the po pulation. This paper will examine residential segregation imposed upon African-Americans and the enormous costs it bears. 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Many people believe global warming is a hoax or a bunch of nonsense evidence constructed by the government; however, it is real and has a major effect on our world. Sea levels will rise, carbon dioxide along with green house gas levels will rise, wild life will die off, and the human race will not be ready for these consequences. Firstly, Earths largest problem, and arguably the most detrimentalRead More Lost Identity Found Essays1862 Words   |  8 Pageschooses to reject Britain in favor of her native India. Certainly-Mary, a nanny and housekeeper, follows her employers to Britain. Even after many years, British lifestyle confuses and alienates her: â€Å"’These English†¦ But aren’t they the limit?† (184). One day, Mary falls ill with a mystery heart condition. Mary states the problem in lay terms: â€Å"’I know what is wrong with me†¦I need to go home.’† (208). Her distance from home had broken her heart; her health problems disappear upon her return to India. ConfrontedRead MoreEssay about Three Approaches to Coping with School Violence2647 Words   |  11 PagesCelestine Prophecy, New York: Warner Books,65–66) Some school critics and statisticians have observed that drug-dealing, vandalism, robbery, and murder have replaced gum-chewing, â€Å"talking out of turn,† tardiness, and rudeness as the most chronic problems afflicting today’s schools. If the intent of this observation is to shock and rattle the public’s sensibilities, it’s working. Of course, some of us may interpret such suggestions as merely dark, stoic, and cynical—â€Å"scare† tactics quite in keeping with